Rapid multiplex high resolution melting method to analyze inflammatory related SNPs in preterm birth

Multiplex amplicon genotyping by high-resolution melting.

High-resolution amplicon melting is a simple method for genotyping that uses only generic PCR primers and a saturating DNA dye. Multiplex amplicon genotyping has previously been reported in a single color, but two instruments were required: a carousel-based rapid cycler and a high-resolution melting instrument for capillaries. Manual transfer of capillaries between instruments and sequential me...

Development of a High-Resolution Melting Method for Screening R188H Polymorphism in XRCC2 Gene

Background: The High Resolution Melting (HRM) method is a new scanning method for detecting unknown changes in DNA and its advantages have persuaded researchers to recruit it as a screening method. Objectives: Here, we developed a HRM method to screen R188H SNP (rs3218536) of XRCC2 and compared the results with a well known PCR-RFLP technique. Materials and Methods: Genomic ...

Rapid Genotyping of Shigella sonnei by Use of Multiplex High-Resolution Melting.

Waleed Mazi, Vartul Sangal, Amir Saeed, Gunnar Sandstrom, François-Xavier Weill, Jun Yu Karolinska Institute, Department of Laboratory Medicine, Division of Clinical Microbiology, Karolinska University Hospital, Huddinge, Stockholm, Sweden; Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Strathclyde, United Kingdom; Faculty of Health and Life Sciences, Nort...

High Resolution Melting Analysis: A Rapid and Accurate Method to Detect CALR Mutations

BACKGROUND The recent discovery of CALR mutations in essential thrombocythemia (ET) and primary myelofibrosis (PMF) patients without JAK2/MPL mutations has emerged as a relevant finding for the molecular diagnosis of these myeloproliferative neoplasms (MPN). We tested the feasibility of high-resolution melting (HRM) as a screening method for rapid detection of CALR mutations. METHODS CALR was...

rapid quantitative determination of gstp1 promoter methylation level using differential high resolution melting analysis (d-hrma)